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1 Agarose and Polyacrylamide Gel Electrophoresis&Andrea M. Guilli 1

2 Internal Labeling of DNA Probes&Ralph Rapley and Bimal D. M. Theophilus 13

3 End-Labeling of DNA Probes&Adrian J. Harwood 17

4 Southern Blotting of Agarose Gels by Capillary Transfer&Ralph Rapley and lan J. Williams 23

5 Restriction Fragment Length Polymorphism&Mohammad S. Enayat 29

6 PCR: Principles, Procedures, and Parameters&Nicola Louise Jones 37

7 Allele-Specific Oligonucleotide PCR&Elaine K. Green 47

8 Long-Range PCR&Peter A. Davies and George Gray 51

9 Analysis of Nucleotide Sequence Variations by Solid-Phase Minisequencing&Anu Wartiovaara and Ann-Christine Syv？nen 57

10 Cycle Sequencing of PCR Products&G. K. Surdhar 65

11 Fluorescent In Situ Hybridization&Sara A. Dyer and Elaine K. Green 73

12 The Protein Truncation Test&Carol A. Hardy 87

13 Mutation Detection in Factor Ⅷ cDNA from Lymphocytes of Hemophilia A Patients by Solid Phase Fluorescent Chemical Cleavage of Mismatch&Naushin H. Waseem, Richard Bagnall, Peter M. Green,and Francesco Giannelli 109

14 Denaturing Gradient Gel Electrophoresis&Yvonne Wallis 125

15 Conformation-Sensitive Gel Electrophoresis&lan J. Williams and Anne C. Goodeve 137

16 SSCP/Heteroduplex Analysis&Andrew J. Wallace 151

17 Cleavase？ Fragment Length Polymorphism Analysis for Genotyping and Mutation Detection&Laura Heisler and Chao-Hung Lee 165

18 Automated Genotyping Using the DNA MassArrayTM Technology&Christian Jurinke, Dirk van den Boom, Charles R. Cantor,and Hubert K？ster 179

19 An Introduction to Bioinformatics&Henry Brzeski 193

Index 209