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1．Impact of Molecular Methods on Clinical Bacteriology&Robert C.George 1

2．Genomic DNA Digestion and Ribotyping&J.Zoe Jordens 17

3．Pulsed-Field Gel Electrophoresis&Mary Elizabeth Kaufmann 33

4．Plasmid Analysis&Alan P.Johnson and Neil Woodford 51

5．DNA Amplification:General Concepts and Methods&Nick A.Saunders and Jonathan P.Clewley 63

6．Arbitrarily Primed PCR Methods for Studying Bacterial Diseases&David Ralph and Michael McClelland 83

7．Genomic Fingerprinting by Application of rep-PCR&Anne M. Ridley 103

8．Molecular Approaches to the Identification of Streptococci&Robert E. McLaughlin and Joseph J.Ferretti 117

9．Pneumococcal Diseases&Anthony M. Smith and Keith P.Klugman 139

10．Molecular Approaches in Mycobacterium tuberculosis and Other Infections Caused by Mycobacterium Species&Madhu Goyal and Douglas B.Young 157

11．Diagnosis and Epidemiology of Diptheria&Androulla Efstratiou,Kathryn H. Engler,and Aruni de Zoysa 191

12．Diagnosis and Epidemiology of Infections Caused by Legionella spp.&Norman K. Fry and Timothy G.Harrison 213

13．Molecular Methods for Haemophilus influenzae&Mark A. Herbert,Derrick Crook,and E. Richard Moxon 243

14．The Impact of Molecular Techniques on the Study of Meningococcal Disease&Martin C. J. Maiden 265

15．Gonorrhea&Catherine A. Ison 293

16．Chancroid&Stephen A. Morse,David L. Trees,and Patricia A. Totten 309

17．Mycoplasma/Ureaplasma Infection&Claire B. Gilroy and David Taylor-Robinson 335

18．Application of Molecular Methods to the Study of Infections Caused by Salmonella spp.&E. John Threlfall,Mike D. Hampton,and Anne M. Ridley 355

19．Cholera&Timothy J. Barrett and Daniel N. Cameron 369

20．Diagnosis and Investigation of Diarrheagenic Escherichia coli&James P. Nataro and Juan Martinez 387

21．Campylobacter Infections:Species Identification and Typing&Janet R. Gibson and Robert J. Owen 407

22．Detection and Typing of Helicobacter pylori&Robert J. Owen and Janet R. Gibson 419

23．Nosocomial Infections Caused by Staphylococci Ariane Deplano and Marc J. Struelens 431

24．Application of Molecular Techniques to the Study of Nosocomial Infection Caused by Enterococci&Teresa M. Coque,Prema Seetulsingh,Kavindra V. Singh,and Barbara E. Murray 469

25．Molecular Approaches for the Detection and Identification of B-Lactamases&David J. Payne and Christopher J. Thomson 495

26．Biochemical and Enzyme Kinetic Applications for the Characte rization of B-Lactamases&David J. Payne and Tony H. Farmer 513

27．B-Lactam Resistance Mediated by Changes in Penicillin-Binding Proteins Christopher G. Dowson and Tracey J. Coffey 537

28．The Application of Molecular Techniques for the Study of Aminoglycoside Resistance&Karen J.Shaw,Frank J.Sabatelli,Linda Naples,Paul Mann,Roberta S. Hare,and George H. Miller 555

29．Molecular Investigation of Glycopeptide Resistance in Gram-Positive Bacteria&Neil Woodford and Jill M. Stigter 579

30．Quinolone Resistance&Janice C. Brown and Sebastian G.B. Amyes 617

31．Resistance to Tetracyclines,Macrolides,Trimethoprim,and Sulfonamides&Marilyn C. Roberts 641

Index 665