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LIST OF CONTRIBUTORS 7

CONSPECTUS 9

PREFACE 11

CONTENTS OF VOLUME 1 13

Optimization in Liquid Chromatography Georges Guiochon 1

Ⅰ．Introduction 1

Ⅱ．Resolution and Efficiency 2

Ⅲ．AnalysiS Time,Flowrate, and Pressure 6

Ⅳ．Flow Velocity and Column Efficiency 7

Ⅴ．What to Optimize in Liquid Chromatography 15

Ⅵ．Equations Used for Optimization 17

Ⅶ．WayS to Achieve 5000 Plates in 5 Minutes 19

Ⅷ．Column Performance at Minimum Inlet Pressure 29

Ⅸ．Critical Minimum Pressure 33

Ⅹ．Achievement of Fastest Analyses 35

Ⅺ．Practical Considerations 39

Ⅻ．Equipment Specifications 43

ⅩⅢ．Conclusions 52

References 54

Liquid Chromatography on Silica and Alumina as Stationary Phases Heinz Engelhardt and Helmut ElgassⅠ．Introduction 57

Ⅱ．Stationary Phases 60

Ⅲ．Role of the Eluent 70

Ⅳ．Effect of Sample Structure on Retention 90

Ⅴ．Anisocratic Analysis 92

Ⅵ Theory of Adsorption Chromatography 101

Ⅶ．Selected Applications 104

Ⅷ．Prospects 106

References 108

Reversed-Phase Chromatography Wayne R．Melander and Csaba HorváthⅠ．Introduction 114

Ⅱ．The Stationary Phase 123

Ⅲ．The Mobile Phase 165

Ⅳ．Effect of Temperature 192

Ⅴ．Theory of Reversed-Phase Chromatography 201

Ⅵ．Modulation of Selectivity by Secondary Equilibria 229

Ⅶ．Physicochemical Measurements 266

Ⅷ．Selected Analytical Applications 279

Notation 299

References 303

Index 321

Erratum 341