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Part Ⅰ.Types of Imaging Reporter Genes 3

1.Fluorescent Reporter Proteins&Robert E.Campbell and Michael W.Davidson 3

2.Revealing Biomolecular Mechanisms Through In Vivo Bioluminescence Imaging&Timothy C.Doyle，Qian Wang，and Christopher H.Contag 41

3.Reporter Gene Imaging with PET/SPECT&June-Key Chung，Joo Hyun Kang，and Keon Wook Kang 70

4.Noninvasive Imaging of Gene Expression with Magnetic Resonance Imaging and Magnetic Resonance Spectroscopy&Mark D.Pagel and James P.Basilion 88

Part Ⅱ.Enhancing Reporter Gene Imaging Techniques 113

5.Multimodality Imaging of Reporter Genes&Pritha Ray and Sanjiv Sam Gambhir 113

6.Cell-Specific Imaging of Reporter Gene Expression Using a Two-Step Transcriptional Amplification Strategy&Marxa L.Figueiredo，Sanjiv Sam Gambhir，Michael Carey，and Lily Wu 127

PartⅢ.Imaging Instrumentations 151

7.Physics，Instrumentation，and Methods for Imaging Reporter Gene Expression in Living Subjects&Craig S.Levin 151

Part Ⅳ.Current Applications of Imaging Reporter Genes 195

8.Reporter Gene Imaging of Cell Signal Transduction&Tarik F.Massoud，Ramasamy Paulmurugan，Carmel Chan，Hua Fan-Minogue，and Sanjiv Sam Gambhir 195

9.Gene Therapy and Imaging of Transgene Expressionin Living Subjects&Shahriar S.yaghoubi and Sanjiv Sam Gambhir 227

10.Imaging Regulation of Endogenous Gene Expression in Living Subjects&Vladimir Ponomarev 239

11.Imaging Cell Trafficking and Immune Cell Activation Using PET Reporter Genes&Evan D.Nair-Gill，Chengyi J.Shu，Isabel J.Hildebrandt，Dean O.Campbell，Owen N.Witte，and Caius G.Radu 258

12.Imaging of Reporter Genes and Stem Cells&Martin G.Rodriguez-Porcel and Sanjiv Sam Gambhir 275

13.Clinical Applications of Reporter Gene Technology&Ivan Penuelas，Shahriar S.Yaghoubi，Felipe Prosper，and Sanjiv Sam Gambhir 297

Index 315