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Chapter 1 Implementation Guide 2

How to Use This Book 2

Teaching Strategies 4

Assessment 6

How to Start a Biotechnology Course 7

Independent Research Projects 10

Chapter 2 Laboratory Skills 12

Activity 2.1 DNA Extraction and Precipitation 12

Activity 2.2 Pipetting 16

Activity 2.3 Kool-Aid Column Chromatography 19

Activity 2.4 Making Solutions 23

Activity 2.5 Titration 28

Activity 2.6 Writing a Standard Operating Procedure 31

Chapter 3 Microbiology and Cell Culture 34

Activity 3.1 Making Microbiology Media 34

Activity 3.2 Disk Diffusion Test （Modified Kirby-Bauer Test） 40

Activity 3.3 Microbes and Health: An Illustration ofKoch's Postulates 44

Activity 3.4 Gram Staining 49

Activity 3.5 Quantifying Bacterial Numbers 52

Activity 3.6 Staining Eukaryotic Cells 55

Chapter 4 DNA Structure and Analysis 58

Activity 4.1 Restriction Site Prediction Using NEBcutter 58

Activity 4.2 Casting Agarose Gels 61

Activity 4.3 Dye Electrophoresis 65

Activity 4.4 Restriction Digestion and Analysis of Lambda DNA 69

Activity 4.5 Forensic DNA Fingerprinting 75

Activity 4.6 Plasmid Mapping 80

Chapter 5 Bacterial Transformation & Plasmid PurificationActivity 5.1 Bacterial Transformation with S3 Plasmid 84

Activity 5.2 Bacterial Transformation with pGLO Plasmid 89

Activity 5.3 Purification of S3 and pGLO Plasmids 93

Activity 5.4 DNA Quantitation 97

Chapter 6 The Polymerase Chain Reaction 102

Activity 6.1 STR PCR Analysis 102

Activity 6.2 GMO Detection by PCR 107

Activity 6.3 Detection of the Human PV92 Alu Insertion 114

Chapter 7 Protein Structure and Analysis 120

Activity 7.1 Protein Quantitation Using the Bradford Assay 120

Activity 7.2 Size Exclusion Chromatography 124

Activity 7.3 GFP Purification by Hydrophobic Interaction Chromatography 127

Activity 7.4 SDS-PAGE of Fish Muscle 132

Activity 7.5 Biofuel Enzyme Assay 137

Activity 7.6 Exploring Bioinformatics with GFP 143

Chapter 8 Immunological Applications 148

Activity 8.1 Ouchterlony Double Immunodiffusion Assay 148

Activity 8.2 Serum Antibody Detection by ELISA 152

Activity 8.3 Quantitative ELISA 156

Activity 8.4 Western Blotting 160

Appendices 167

Appendix Ⅰ: Debate Activity 167

Appendix Ⅱ: Technical Writing Rubric 169

Appendix Ⅲ: Semilog Graph Paper 170

Appendix Ⅳ: Activities Sorted By Skill Level 171

Appendix Ⅴ: Pacing Guides 172

Appendix Ⅵ: Purchasing Guide 174

Appendix Ⅶ: Trademarks and Legal Notices 187