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Section Ⅰ．Techniques for Characterization of Proteins (Procedures and Interpretations) 1

1．Electrophoresis＆ARNE TISELIUS 2

1A．Paper Electrophoresis＆A．HELGE F．LAURELL 21

2．Ultracentrifugation，Diffusion，and Viscometry＆H．K．SCHACHMAN 32

3．Infrared Spectrophotometry＆D．L．WOOD 104

4．X-Ray Diffraction of Protein Crystals＆F．H．C．CRICK 127

5．Light-Scattering Measurements＆M．BIER 147

6．Flow Birefringence＆W．F．H．M．MOMMAERTS 166

7．Fluorescence Techniques for the Enzymologist＆DONALD J．R．LAURENCE 174

8．The Solubility Diagram as a Criterion of Protein Homogeneity＆ROGER M．HERRIOTT 212

9．Determination of Amino Acid Sequence in Proteins＆R．R．PORTER 221

10．The Pipsyl Method for Amino Acid Sequences in Proteins＆MILTON LEVY 238

11．Methods for Investigating the Essential Groups for Enzyme Activity＆H．FRAENKEL-CONRAT 247

Section Ⅱ．Techniques for Metabolic Studies 271

12．Techniques for the Assay of the Respiratory Enzymes＆BRITTON CHANCE 273

13．Use of Artificial Electron Acceptors in the Study of Dehydrogenases＆J．H．QUASTEL 329

14．Study of soil Metabolism Using the Perfusion Technique＆J．H．QUASTEL and P．G．SCHOLEFIELD 336

15．Methods for Study of the Hill Reaction＆WOLF VISHNIAC 342

16．Methods for Measurement of Nitrogen Fixation＆R．H．BURRIS and P．W．WILSON 355

17．Micromethods for the Assay of Enzymes＆O．H．LOWRY 366

18．Histochemical Methods for Enzymes＆GEORGE GOMORI 381

19．Electron Microscopy of Cellular Constituents＆FRITIOF S．SJOSTRAND 391

Section Ⅲ．Techniques for Isotope Studies 423

20．The Measurement of Radioisotopes＆DANIEL STEINBERG and SIDNEY UDENFRIEND 425

21．The Measurement of Stable Isotopes＆ANTHONY SAN PIETRO 473

22．The Synthesis and Degradation of Isotopically Labeled Carbohydrates and Carbohydrate Intermediates＆S．ABRAHAM and W．Z．HASSID 489

23．Determination of Isotopic Carbon Patterns in Carbohydrate by Bacterial Fermentation＆I．A．BERNSTEIN and HARLAND G．WOOD 561

24．Isotopic Experimentation with Intermediates of the Tricarboxylic Acid Cycle＆H．E．SWIM and M．F．UTTER 584

25．Synthesis and Degradation of Isotopically Labeled Glyeolic，Glyoxylic，and Oxalic Acids＆KATHARINE F．LEWIS and SIDNEY WEINHOUSE 609

26．Purines and Pyrimidines＆EDWARD D．KORN 615

27．Biosynthesis of Protoporphyrin＆DAVID SHEMIN 643

28．Methods for Chemical Synthesis，Isolation，and Degradation of Labeled Compounds as Applied in Metabolic Studies of Amino Acids and Proteins＆DAVID M．GREENBERG and MORTON ROTHSTEIN 652

29．Synthesis and Degradation of Labeled Steroids＆K．BLOCH 732

30．Labeled Compounds Related to Methyl Group Biosynthesis and Transfer＆J．A．STEKOL 751

31．The Synthesis of Labeled Sulfur Compounds＆HAROLD TARVER 769

32．The Synthesis and Degradation of Carbon-Labeled Fatty Acids and Related Compounds＆H．S．ANKER 779

33．Labeled Compounds in the Study of Phospholipid Metabolism＆CAMILLO ARTOM 809

34．Preparation and Analysis of Labeled Coenzymes＆SIDNEY P．COLOWICK and NATHAN O．KAPLAN 840

35．Synthetic and Analytic Procedures Involving I131-Labeled Compounds＆ALVIN TAUROG and I．L．CHAIKOFF 856

36．Intermediates of Photosynthesis:Isolation and Degradation Methods＆A．A．BENSON and M．CALVIN 882

37．O18 Containing Phosphate and Related Compounds＆MILDRED COHN 905

AUTHOR INDEX 915

SUBJECT INDEX 941