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Section A - Cell organization 1

A1 Prokayotes 1

A2 Eukaryotes 4

Abbreviations 8

Preface 10

A3 Microscopy 10

A4 Cellular fractionation 15

Section B - Amino acids and proteins 19

B1 Amino acids 19

B2 Acids and bases 23

B3 Protein structure 27

B4 Myoglobin and hemoglobin 36

B5 Collagen 43

B6 Protein putification 50

B7 Chromatogyaphy of proteins 54

B8 Electrophoresis of proteins 58

B9 Protein sequencing and peptide synthesis 63

Section C - Enzymes 69

C1 Introduction to enzymes 69

C2 Thermodynamics 76

C3 Enzyme kinetics 81

C4 Enzyme inhilition 87

C5 Regulation of enzyme activity 90

Section D - Antibodies 97

D1 The immune system 97

D2 Antibody structure 101

D3 Polyclonal and monoclonal antibodies 105

D4 Antibody synthesis 107

D5 Antibodyies as tools 112

Section E - Membranes 117

E1 Membrane lipids 117

E2 Membrane protein and carbohydrate 124

E3 Membrane transport,small molecules 131

E4 Membrane transport:macromolecules 136

E5 Signal transduction 141

Section F - DNA structure and replication 147

F1 DAN structure 147

F2 Chromosomes 153

F3 DNA replication in bacteria 157

F4 DNA replication in eukaryotes 162

Section G - RNA synthesis and processing 167

G1 RNA structure 167

G2 Transcription in prokaryotes 169

G3 The lac operon 173

G4 The trp operon 177

G5 Transcription in eukaryotes:an overview 181

G6 Transcription of protein-coding geies in eukaryotes 183

G7 Regulation of transcription by RNA PolⅡ 187

G8 Processing of eukaryotic pre-mRNA 195

G9 Ribosomal RNA 203

G10 Transfer RNA 209

Section H - Protein symthesis 215

H1 The genetic code 215

H2 Translation in prokaryotes 219

H3 Translation in eukaryotes 227

H4 Protein targeting 230

H5 Protein glycosylation 238

I1 Restriction enzymes 243

Section I - Recombinant DNA technology 243

I2 Nucleic acid hybridization 248

I3 DAN cloning 251

I4 Viruses 256

I5 DNA sequencing 260

I6 Polymerase chain reaction 263

Section J - Carbohydrate metabolism 267

J1 Monosaccharides and disaccharides 267

J2 Polysaccharides and oligosaccharides 274

J3 Glycolysis 278

J4 Gluconeogenesis 289

J5 Pentose phosphate pathway 298

J6 Glycogen metabolism 302

J7 Control of glycogen metabolism 305

Section K - Lipid metabolism 311

K1 Structures and roles of fatty acids 311

K2 Fatty acid breakdown 315

K3 Fatty acid synthesis 322

K4 Triacylglycerols 328

K5 Cholesterol 333

K6 Lipoproteins 339

Section L - Respiration and energy 343

L1 Citric acid cycle 343

L2 Electron transport and oxidative phosphorylation 347

L3 Photosynthesis 359

Section M - Nitrogen metabolism 369

M1 Nitrogen fixation and assimilation 369

M2 Amion aciid metabolism 373

M3 The urea cycle 380

M4 Hemes and chlorophylls 386

Section N - Cell specialization 391

N1 Muscle 391

N2 Cilia and foagella 397

N3 Nerve 400

Futther reading 405

Index 413