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Chapter 1 Basics of Physiological-Pharmacological Experimentation 1

Unit 1 Objectives and Requirements of Physiological-Pharmacological Experiment 1

Unit 2 Process of Experiment Data and Production of Experiment Reports 3

Unit 3 Requirements and Safety Rules in Laboratory 7

Unit 4 Operation of Biological Data Acquisition System RM-6240 15

Chapter 2 General Knowledge of Physiological-Pharmacological Experimentation 22

Unit 1 Fundamental Principle of Physiological-Pharmacological Experi-mentation 22

Unit 2 Preliminary Tests in Physiological-Pharmacological Experimentation 28

Unit 3 Statistical Analysis Most Often Used in Scientific Activities 31

Unit 4 Statistic Analysis Using Excel 35

Chapter 3 Basic Operations on Experimental Animals 44

Unit 1 Fundamentals of Animal Experiments 44

Unit 2 Operations on Experimental Animals 48

Unit 3 Gender Identification and Labeling the Animals 51

Unit 4 Narcotics and Animal Narcosis 55

Unit 5 Sample Collection and Bleeding 57

Unit 6 Drug Administration for Laboratory Animals 60

Chapter 4 Physiological-Pharmacological Experiments 66

Experiment 1 Generation and Conduction of Compound Action Potential 66

Experiment 2 Actions of Antiepileptic and Anticonvulsants in Mouse Models 70

Experiment 2-1 Effect of Drugs on Electric Convulsive Shock 70

Experiment 2-2 Effect of Drugs on Convulsion Induced by Central Stimulants 72

Experiment 3 Actions of Analgesics in Mouse Models 74

Experiment 3-1 Analgesic Effect Assayed by Hot-Plate Model 74

Experiment 3-2 Analgesic Effect Assayed Using Chemical-Stimulation Model 76

Experiment 4 Influence of Stimulus on the Contraction of Skeletal Muscle 78

Experiment 5 Determination of ABO Blood Group 81

Experiment 6 Effects of Physical and Chemical Factors on Blood Coagulation 83

Experiment 7 Recording of Electrocardiogram in Toads 85

Experiment 8 Effects of Chemical Agents on the Activity of the Isolated Toad Heart 88

Experiment 9 Regulation of Artery Blood Pressure 91

Experiment 10 Measurement of Human Blood Pressure 94

Experiment 11 Toxic Effects of Cardiac Glycosides on Heart 97

Experiment 12 Effect of Drugs on Isolated Intestinal Smooth Muscles 101

Experiment 13 Regulation of Respiratory Movements 103

Experiment 14 Experimental Pulmonary Edema in Rabbits 106

Experiment 15 Factors and Drugs Influencing Urine Output 110

Experiment 16 Drug Actions Influenced by Dosage Forms and Delivery Routes 113

Experiment 17 Determination of 50 3.485388E-317ffective Dose of Pentobarbital 115

Experiment 18 Measurement of Concentration-Time Profile of Metronida-zole 118

Experiment 19 Calculation of the Pharmacokinetic Parameters 122

Experiment 20 Intoxication and Detoxification of Organophosphate Agents 127

Experiment 21 Effect of Glucocorticoids on Intraabdominal Capillary Permeability in Mice 130

Experiment 22 Effect of Anti-inflammatory Agents on Tumescence in Rat Model 133

Experiment 23 In-Vitro Experiment of Antibacterial Actions 136

Chapter 5 Expanded Experiments 139

Experiment 1 Extraction of Genomic DNA in Peripheral Blood 139

Experiment 2 Extraction of mRNA from Liver Tissues 142

Experiment 3 Assessment of Gene Transcription Level by Real-Time PCR 145

Experiment 4 Assessment of Protein Expression by Western-Blot 148

Experiment 5 Isolation and Culture of Mouse Hepatocytes 152

Appendix 156

Table 1 Physiological parameters of experimental animals 156

Table 2 Age,body weight and lifetime of adult experimental animals 157

Table 3 Formularies of commonly used saline solution 157

Table 4 Bleeding methods for experimental animals 158

Table 5 Safe and lethal bleeding volume of experimental animals 158

Table 6 Scaling factors for cross-species dose calculation 159

Table 7 Application route and dose of commonly used anaesthetics 160

参考文献 161