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Part 1 Basic Knowledge in Organic Chemistry Laboratory 1

1.1 Rules in laboratory 1

1.2 Prevention and treatment of the common accidents in laboratory 1

1.3 Requirements of preparation，record and report of experiments 7

1.4 Common lab equipment and apparatus 8

1.5 Waste disposal 10

Part 2 Basic Experimental Techniques 12

2.1 Preparation of organic compounds 12

2.2 The determination of physical constants of organic compounds 16

2.3 Separation and purification technology of organic compounds 25

2.4 Spectral analysis technology of organic compounds 46

Part 3 Experiments for Preparation of Organic Compounds 57

3.1 Preparation of cyclohexene 57

3.2 Preparation of anhydrous ethanol 59

3.3 Preparation of n-butyl bromide 61

3.4 Preparation of n-butyl ether 63

3.5 Preparation of acetophenone 66

3.6 Preparation of aspirin 68

3.7 Preparation of cinnamic acid 71

3.8 Preparation of n-butyl acetate 73

3.9 Preparation of isoamyl acetate 76

3.10 Synthesis of glycerol monostearate 78

3.11 Preparation of skin care cosmetic 81

3.12 Preparation of detergent 84

Part 4 Extraction，Separation and Identification of Organic Compounds 88

4.1 Distilling 95％ethanol and measuring the boiling point 88

4.2 Recrystallization of benzoic acid 89

4.3 Purification of benzaldehyde 90

4.4 Isolation of cafieine from tea leaves 92

4.5 The extraction of limonene from orange peels 94

4.6 Separation of A and B by thin-layer chromatography 96

4.7 Separation of spinach pigments by column chromatography 97

4.8 Preparation and resolution for optical isomers of tris（ethylenediamine）cobalt salts 101

4.9 Identification of sugar 105

4.10 Identification of amino acids and proteins 107

Reference 110

Appendix：Experimental Graphs and Properties of Organic Solvents 111

Ⅰ．Distilling 95％ethanol and measuring the boiling point 111

Ⅱ．Recrystallization of benzoic acid 113

Ⅲ．Isolation of caffeine from tea leaves and sublimation 116

Ⅳ．Synthesis，isolation and purification of n-butyl acetate 117

Ⅴ．Physical constant 119