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PARTⅠ Laying the Foundation 1

1 An Overview of Biotechnology 3

2 Cell Properties and Processes 45

3 Genes，Genetics，and Geneticists 73

4 An Overview of Molecular Biology 97

5 Recombinant DNA Technology 137

PARTⅡ Classroom Activities 169

A.DNA Structure and Function 171

6 Student Activity：Constructing a Paper DNA Model 172

7 Student Activity：DNA Replication 174

8 Student Activity：From Genes to Proteins 176

Student Activity：Making Antisense：Regulating Gene Expression with RNA 179

9 Student Activity：Sizes of the Escherichia coli and Human Genomes 182

10 Student Activity：Extraction of Bacterial DNA 185

B.Manipulation and Analysis of DNA 187

11 Student Activity：DNA Scissors 188

12 Student Activity：DNA Goes to the Races 190

13 Student Activity：Restriction Analysis of Lambda DNA 194

14 Student Activity：Recombinant Paper Plasmids 199

15 Student Activity：Restriction Analysis Challenge 202

16 Student Activity：Detection of Specific DNA Sequences：PartⅠ.Fishing for DNA 205

Student Activity：Detection of Specific DNA Sequences：PartⅡ.Combining Restriction and Hybridization Analysis 207

Student Activity：Detection of Specific DNA Sequences：PartⅢ.Southern Hybridization 209

17 Student Activity：Paper PCR 210

18 Student Activity：DNA Sequencing：The Terminators 214

Reading：Chain Terminators as Antiviral Drugs 218

C.Transfer of Genetic Information 221

19 Student Activity：Transformation of Escherichia coli 222

20 Student Activity：Conjugative Transfer of Antibiotic Resistance in Escherichia coli 225

Reading：Gene Transfer and the Spread of Antibiotic Resistance 228

21 Student Activity：Transduction of an Antibiotic Resistance Gene 234

22 Student Activity：Agrobacterium tumefaciens：Nature’s Plant Genetic Engineer 238

D.Molecular Biology and Genetics 241

23 Student Activity：An Adventure in Dog Hair，Part Ⅰ 242

24 Student Activity：An Adventure in Dog Hair，Part Ⅱ：Yellow Labs 246

25 Student Activity：Human Molecular Genetics 250

Reading：Molecular Genetics of Cancer 258

26 Student Activity：Genetics in Action 264

E.Genomics 271

27 Student Activity：Comparing Genomes 272

Exercise 1：STRs Can Cause an RFLP 278

Exercise 2：PCR Can Reveal Differences at Microsatellite Loci 279

Reading：Mitochondrial DNA 281

28 Student Activity：Forensic DNA Typing 283

Exercise 1.A Mix-Up at the Hospital 285

Exercise 2.A Paternity Case 285

Exercise 3.The Case of the Bloody Knife 286

Reading：DNA Forensics：An Archaeological Case 289

29 Student Activity：Mapping a Disease Gene 290

Reading：The Human Genome Project：Its Science，Applications，and Issues 299

30 Student Activity：Microarray Analysis of Genome Expression 303

Reading：Personal Genomics 306

F.Bioinformatics and Evolutionary Analysis of Proteins 309

31 Student Activity：Testing for Amylase Activity 310

32 Student Activity：Electrophoresis of Amylase Samples 315

33 Student Activity：Constructing an Amylase Evolutionary Tree 318

34 Student Activity：Exploring Bioinformatics 322

PART Ⅲ Societal Issues 329

Introduction to Societal Issues 331

35 Student Activity：Facing the Forces：Science，Technology，and Society 332

Reading：The Nature of Science 338

36 Student Activity：Analyzing the Issues of Biotechnology：Cloning 341

Reading：Are Bioengineered Foods Safe？ 352

37 Student Activity：The Risks of Technology：Perception，Reality，or Both？ 356

Reading：Biotechnology Regulation in the United States 368

38 Student Activity：Making Decisions When There Is No Right Answer 372

39 Student Activity：Analyzing Ethical Dilemmas in Genetic Testing 377

Reading：Science，Law，and Politics 381

40 Student Activity：Analyzing Ethical Dilemmas in Gene Therapy 384

PART Ⅳ Appendixes 389

A Worksheets 390

B Laboratory Biosafety 453

C Aseptic Technique 455

Glossary 457

Index 469